Key result
Patch-clamp protocol enables robust measurement of action potentials and funny current in mouse SAN cells.
Why the study?
Detailed protocols for measuring ionic currents and action potentials in murine sino-atrial node cells using patch-clamp techniques are needed to investigate cardiac pacemaker function.
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Provides detailed protocols for using patch-clamp techniques to investigate ion channel function in murine sino-atrial node cells.
Cardiac pacemaker cells of the sino-atrial node are responsible for the initiation of the heart beat and express an array of ion channels.The patch-clamp technique is the gold standard method for investigating the function of ion channels expressed in electrically active cells.Conventional whole-cell and perforated patch-clamp techniques can be used to investigate ionic currents in the voltage-clamp mode and changes in membrane potential (e.g., action potential) in the current-clamp mode.Here, we provide details of protocols used to measure spontaneous and triggered action potentials and whole-cell funny current If (HCN4) in single cardiomyocytes isolated from the mouse sino-atrial node (SAN).
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Aziz et al. (2020) studied this question. Patch-clamp technique was evaluated. This paper provides detailed protocols for measuring spontaneous and triggered action potentials and whole-cell funny current in single cardiomyocytes isolated from the mouse sino-atrial node.
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