Why the study?
Information regarding the formation and thickness of cryo-sections is important for adequate interpretation of cryo-sectioned biological material.
Provides methodological insights into the formation of cryo-sections of biological material, showing they form partly by fracturing.
Warrants caution interpreting cryo-section ultrastructure in research; leaves open validation in human clinical samples.
Information regarding the formation and the thickness of cryo-sections is of importance for an adequate interpretation of cryo-sectioned biological material. In this study we have taken advantage of the regular arrangement of filaments in myofibrils in an analysis of these matters. It is concluded that the sections are formed partly by fracturing in a way similar to that visualized in replicas made by the freeze-fracturing and -etching procedure.
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Thornell et al. (1975) studied this question. Cryo-sectioning was evaluated on Formation and thickness of cryo-sections.
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