Key result
Pentacistronic retroviral vectors successfully co-express five proteins while maintaining viral titres comparable to shorter vectors.
Why the study?
Efficient co-expression of multiple proteins using pentacistronic retrovirus vectors and the impact of genome size on vector stability were not well characterized.
Pentacistronic retrovirus vectors up to approximately 7.9 kb can be constructed using 2A sequences and IRES elements to efficiently co-express five proteins with titres comparable to shorter vectors.
Multicistronic retroviral vectors support multi-protein co-expression; extends basic vector engineering while leaving therapeutic applications open.
The picornavirus foot-and-mouth disease virus 2A sequence was combined with three different internal ribosome entry segments to construct and characterize three independent pentacistronic retroviruses of different sizes. Efficient co-expression of the five proteins was successful and titres obtained for these pentacistronic virus vectors (final genome size approximately 7.9 kb) were comparable to those of vector systems with shorter genomes. Other vectors constructed that exceeded the genome length of the wild-type virus suffered frequent deletions.
No takes yet. Share an insight, caveat, or question.
Felipe et al. (2003) studied this question. Pentacistronic retrovirus vectors vs. Vector systems with shorter genomes was evaluated on Co-expression of five proteins and viral titres. Pentacistronic retrovirus vectors successfully co-expressed five proteins and achieved viral titres comparable to vector systems with shorter genomes.
Synapse has enriched 5 closely related papers on similar clinical questions. Consider them for comparative context: