A novel picornavirus vector design replacing conserved noncoding IRES sequences with foreign coding sequences significantly improves the genetic stability and expression of heterologous inserts.
Supports stable picornavirus vector engineering; leaves open in vivo efficacy and clinical translation.
In many respects, picornaviruses are well suited for their proposed use as immunization vectors. However, their inherent genetic instability hinders application for prophylactic purposes. We demonstrate the improved expression and stability of a heterologous insert through a novel vector design strategy that partially replaces noncoding regulatory sequences with coding sequences for foreign gene products.
No takes yet. Share an insight, caveat, or question.
Dufresne et al. (2002) studied this question.
Synapse has enriched 5 closely related papers on similar clinical questions. Consider them for comparative context: