Key result
In older men, blockade of KCa and KATP channels attenuated cutaneous vasodilation during rest and exercise in the heat, respectively, and these effects were NOS dependent (P≤0.05).
Why the study?
Does blockade of KCa and KATP channels with NOS inhibition alter cutaneous vasodilation and sweating in older men during heat stress?
Does blockade of KCa and KATP channels with NOS inhibition alter cutaneous vasodilation and sweating in older men during heat stress?
p-value: p=≤0.05
In older men, KCa and KATP channels regulate cutaneous vasodilation during rest and exercise in the heat in a NOS-dependent manner.
Our objective in this study was to examine the separate and combined effects of potassium (K+) channels and nitric oxide synthase (NOS) on cutaneous vasodilation and sweating in older men during rest and exercise in the heat. In 13 habitually active men (61 ± 4 yr), cutaneous vascular conductance and local sweat rate were assessed at six dorsal forearm skin sites continuously perfused with either 1) lactated Ringer (control), 2) 10 mM NG-nitro-l-arginine methyl ester (l-NAME, NOS inhibitor), 3) 50 mM tetraethylammonium (TEA; Ca2+-activated K+ channel blocker), 4) 5 mM glybenclamide (GLY; ATP-sensitive K+ channel blocker), 5) 50 mM TEA + 10 mM l-NAME, and 6) 5 mM GLY + 10 mM l-NAME via microdialysis. Participants rested in non-heat stress (25°C) and heat stress (35°C) conditions for ∼60 min each, followed by 50 min of moderate-intensity cycling (∼55% V̇o2peak) and 30 min of recovery in the heat. During rest and exercise in the heat, l-NAME, TEA + l-NAME, and GLY + l-NAME attenuated CVC relative to control (all P ≤ 0.05), although l-NAME was not different from TEA + l-NAME or GLY + l-NAME (all P > 0.05). TEA attenuated CVC during rest, whereas GLY attenuated CVC during exercise (both P ≤ 0.05). Additionally, whereas neither l-NAME nor TEA altered sweating throughout the protocol (all P > 0.05), combined TEA + l-NAME attenuated sweating during exercise in the heat ( P ≤ 0.05). We conclude that in habitually active older men blockade of KCa and KATP channels attenuates cutaneous vasodilation during rest and exercise in the heat, respectively, and these effects are NOS dependent. Furthermore, combined NOS inhibition and KCa channel blockade attenuates sweating during exercise in the heat.
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McGarr et al. (2019) studied Healthy habitually active older men (n=13). KCa and KATP channel blockade with NOS inhibition (TEA, GLY, l-NAME) vs. Lactated Ringer (control) was evaluated on Cutaneous vascular conductance and local sweat rate (p=≤0.05). In older men, blockade of KCa and KATP channels attenuated cutaneous vasodilation during rest and exercise in the heat, respectively, and these effects were NOS dependent (P≤0.05).
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