Early Immune Disorders and Neutrophil Activation Induced By Childhood Obesity; CD66b and Myeloperoxidase

Neutrophils are major source of inflammatory and pro-inflammatory cytokines which play an important role in obesity related cardio-metabolic complications.Little is known about neutrophil activation markers in childhood obesity.Therefore, we investigated the hypothesis that, the component of the innate immune system is activated in childhood obesity through assessment of the expression of CD66b as a specific marker of neutrophil activation and plasma MPO which is a product of activated neutrophil.Ninety children aged 5-15 years (60 obese and 30 age and sex matched healthy controls) were included in the present study.All were subjected to anthropometric measurements, routine laboratory investigations, plasma myeloperoxidase (MPO), complete blood picture (CBC) with examination of peripheral blood smears stained with Giemsa.Surface expression of degranulation marker CD66b on neutrophil surface from peripheral blood was assessed through flow cytometry.We observed that, total WBCs count and total neutrophils count, hs-CRP, and MPO were significantly increased in obese children compared to control group.Although Cd66b expression showed no statistically significant difference, mean fluorescent intensity (MFI) of CD66b positive cells was significantly increased in obese.Our data showed that CD66b, MFI and MPO are major neutrophil activation markers.It also proved that, the innate immune system is activated in childhood obesity.