Tissue engineering and 3D cell cutler involving the use of three-dimensional scahffold for cell and tissue cultural ,is a promising technique for establishing in vitro culture models that mimc in vivo environment . In vitro models present ethical and cost advantages over in vivo models, and allow for tissue devolpmint.alginate , aliner polysaccharide derived from brown algae .has properties that make it a favorable materials as a 3D extracellular matrix for in vitro cell and tissue models and some of sodium Hydrogyl materials are higly appealing as 3D scaffold for in vitro 3D cell cutlre and tissue culture due their ability to mimic the physical properties native tissue as well as their high porosity for effcient diffusion of proteins and nutrients Alginate hydrogel is widely ysed in tissue engineering application due to its desirable propertis as Biomaterials .
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Aljohani et al. (2017) studied this question.
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