Key result
Acute chemerin reduces left ventricular developed pressure by ~35% in isolated rat hearts.
Why the study?
Chemerin is a novel adipokine expressed in the cardiovascular system with unclear effects on cardiac parameters and gene expressions in the heart.
Answers are grounded in this paper and cite their sources.
May impair cardiac performance in rats; hypothesis-generating for chemerin's role in human cardiovascular disease.
Background: Chemerin is a novel chemoattractant adipokine expressed in cardiovascular system, and its receptor has been detected in the epicardial adipose tissue. Aims: To determine the effects of chemerin on the cardiac parameters and gene expressions in the isolated perfused rat heart. Study Design: Animal experiment. Methods: The hearts were retrogradely perfused with Langendorff technique to measure the cardiac parameters. The experimental groups were acutely treated with 10, 100, and 1000 nM doses of chemerin. Another group was given 10 μM L-nitric oxide synthase inhibitor for 5 min before 1000 nM chemerin administration. The real-time polymerase chain reaction was performed for detecting the expression of target genes. Results: (max 31.3 Δ%, p<0.001), which are the indexes of cardiac contractile force. In addition, 1000 nM chemerin reduced the coronary flow (max 31 Δ%, p<0.001). N(W)-nitro-L-arginine methyl ester antagonized the negative inotropic effect of chemerin on contractility. Chemerin induced a 2.16-fold increase in endothelial nitric oxide synthase mRNA and increased the cyclic guanosine monophosphate levels (p<0.001) but decreased the PI3Kγ gene expression (1.8-fold, p<0.001). Furthermore, all doses of chemerin decreased the CaV1.2 gene expression (1.69-fold, p<0.001). Conclusion: Acute chemerin treatment induces a negative inotropic action with the involvement of nitric oxide pathway, CaV1.2, and PI3Kγ on isolated rat heart.
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Kutlay et al. (2018) studied Isolated perfused rat heart. Chemerin vs. Krebs Henseleit solution (control) was evaluated on Left ventricular developed pressure (LVDP) (35.33 Δ% maximum reduction, p=<0.001). Acute treatment with 1000 nM chemerin significantly reduced left ventricular developed pressure by a maximum of 35.33% and coronary flow by 31% in isolated perfused rat hearts.
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