Esaxerenone attenuates renal injury by inhibiting NR3C2-mediated inflammatory and fibrotic pathways

Figures

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  Figure 1.. The process of the experiment.

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  Figure 2.. Esaxerenone alleviates the renal pathological damage induced by high-salt diet plus aldosterone (A) HE and Masson staining demonstrate histological changes in the kidney of each group. (Scale bar = 100 μm). (B and C) The quantification of pathological score and collagen volume fraction (D) Western blot shows the expression of KIM-1 in the kidneys of each group. (E) The quantification of KIM-1. Every value represents the mean ± SD, n = 3 denotes the number of randomly selected animals from each group. *p < 0.05, vs. CON group, #p < 0.05, vs. ALD+HSD group.

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  Figure 3.. Esaxerenone improves high-salt diet plus aldosterone-induced renal fibrosis. (A) Immunohistochemistry shows the expression of α-SMA, Vimentin and collagen I in the kidneys of each group (Scale bar = 100 μm). (B–D) The quantification of α-SMA, Vimentin and collagen I. (E) Western blot shows the expression of α-SMA, Vimentin, and collagen I in the kidneys of each group. (F–H) The quantification of α-SMA, Vimentin and collagen I. (I) Western blot shows the expression of TGF-β1, p-Smad2, Smad2, p-Smad3, and Smad3 in the kidneys of each group. (J–L) The quantification of TGF-β1, Smad2, and Smad3. Every value represents the mean ± SD, n = 3 denotes the number of randomly selected animals from each group. *p < 0.05, vs. CON group, #p < 0.05, vs. ALD+HSD group.

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  Figure 4.. Esaxerenone alleviates high-salt diet plus aldosterone-induced renal inflammatory response. (A) Western blot shows the expression of TLR4 and NF-κB in the kidneys of each group. (B–C) The quantification of TLR4 and NF-κB. (D) Immunohistochemistry shows the expression of F4/80, IL-6, TNF-α, and NF-κB in the kidneys of each group (Scale bar = 50 μm). (E–H) The quantification of F4/80, IL-6, TNF-α, p-NF-κB, and NF-κB. (I) Western blot shows the expression of IL-6, IFN-γ and TNF-α in the kidneys of each group. (J–L) The quantification of IL-6, IFN-γ and TNF-α. Every value represents the mean ± SD, n = 3 denotes the number of randomly selected animals from each group. *p < 0.05, vs. CON group, #p < 0.05, vs. ALD+HSD group.

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  Figure 5.. Esaxerenone may inhibit renal inflammation and fibrosis induced by high-salt diet plus aldosterone through suppressing the activation of MR. (A) Western blot shows the nucleus and total protein of NR3C2 and SGK-1 expression in the kidneys of each group. (B–D) The quantification of total and nucleus protein of NR3C2 and SGK-1. (E–J) Correlation analysis of IL-6, Collagen I, KIM-1, TLR4, TGF-β1, and NR3C2, IL-6, and Collage I in kidney of rats. Every value represents the mean ± SD, n = 3 denotes the number of randomly selected animals from each group. *p < 0.05, vs. CON group, #p < 0.05, vs. ALD+HSD group.