An Investigation of Serum Lipoprotein Disc Electrophoresis : Ii. A Simple Method of Human Serum Lipoprotein Disc Electrophoresis and Identification of the Resolved Lipoproteins

An integrated and simplified method for acrylamide gel Disc electrophoresis was devised in this study based on results obtained by the previous study where general behavior of human serum lipoproteins in various acrylamide gel systems was investigated. Acrylamide gel concentrations employed in this method were 3.75% for separating gel, 2.5% for spacer gel and 3.3% for sample gel. For lipoprotein staining, serum samples were mixed with a sample gel solution in which Sudan Black B in ethylene glycol and buffered acrylamide solution with a combined catalyzer (TEMED-riboflavin) were included. Identification of lipoprotein bands on acrylamide gel columns was made by means of a combination of paper electrophoretic, acrylamide gel Disc electrophoretic and immunological techniques. It was established that human serum lipoproteins were sharply resolved and were arranged in the order of alpha, beta, and pre-beta lipoproteins from the anodal to cathodal end of the column. This method could be useful in clinical laboratories as well as in research laboratories for identification of human serum lipoprotein abnor-malities.