Chemical composition and antimicrobial effects of Thymus daenensis on Helicobacter pylori

High occurrence of resistance in the Helicobacter pylori strains of human clinical samples caused medical practitioners to found a good alternative therapeutic choice.High phenolic contents of the Thymus daenensis essential oil covered its high antimicrobial effects.The present investigation was done to study the chemical composition and anti-H.pylori effects of T. daenensis essential oil.Aerial parts of the T. daenensis were collected from the Yasuj city and transferred to the laboratory.Essential oil was extracted using the Clevenger apparatus.Chemical components of T. daenensis was identifi ed using the GC-mass analyzer.Anti-H.pylori effects of T. daenensis was determined using the disk diffusion method.Twelve (99.24%) chemical components were totally identifi ed in the essential oil of the T. daenensis.Thymol (42.81%), gamma-Terpinene (20.39%) and para-Cymene (9.72%) were the most commonly identifi ed chemical components.Distribution of beta-Myrcene, D-Limonene, beta-Pinene and Terpinen-4-ol were moderate.H. pylori strains harbored the highest levels of sensitivity against tetracycline and ampicillin antibiotics, while those of resistance were seen for the erythromycin, clarithromycin and metronidazole antibiotics.Inhibition zone diameter increased from 0 to 18.6±0.5mm which represented dose depended anti-H.pylori effects of T. daenensis essential oil.Extensive production of T. daenensis full from thymol, gamma-Terpinene and para-Cymene will help researchers to formulate an effective antibiotic agent for treatment of the cases of H. pylori-infection.