G-CSF-producing renal cell carcinoma characterized by IL-8-induced marked neutrophil infiltration to tumor tissue

Although G-CSF-producing tumor is well-known, co-production of IL-8 by such tumor and IL-8-induced neutrophil chemotaxis to tumor tissue have not been recognized.A 65-year-old male was diagnosed with papillary renal cell carcinoma accompanied by marked intratumoral neutrophil infiltration after left radical nephrectomy.At presentation, laboratory tests showed a white blood cell count of 47.8×10 9 /l with 84.1% neutrophils and a high CRP of 18.3 mg/dl.Serum concentrations of G-CSF, IL-6, and IL-8 were elevated to 143 pg/ml, 474 pg/ml, and 1,260 pg/ml, respectively.After the operation, the white cell count and serum concentrations of these cytokines returned to normal levels.Real-time quantitative PCR of the tumor tissue revealed high gene expressions of G-CSF and IL-8, which appeared to be the cause of the leukocytosis and intratumoral neutrophil infiltration.