In Vitro and in Vivo Densitometric Analysis of DNA Content and Chromatin Texture in Nuclei of Tumor Cells under the Influence of a Nano composite and Magnetic Field

DNA content and chromatin texture of interphase nuclei in MCF-7 and Guerin carcinoma cells that are sensitive or resistant to chemotherapeutic agents in the setting of the treatment with cisplatin, nanocomposite and static magnetic field have been analyzed. The results obtained indicate that chemotherapy agents and physical factors both in vitro and in vivo cause the increase in the DNA content. Tumor exposure to chemotherapy agents, nanocomposite and magnetic field causes the increase in the percentage of heterochromatin in nuclei of resistant tumor cells compared with sensitive ones, especially under the influence of combination of nanocomposite with static magnetic field. Probably it suggests the specific effects of combination of nanocomposite with magnetic field on cell chromatin depending on the changes in the strength of the DNA-histone interactions.