Key result
Nimodipine and flunarizine alleviate post-SAH vasospasm without significantly altering Cav3.1 expression.
Why the study?
Delayed cerebral vasospasm after subarachnoid hemorrhage is a common complication with unclear mechanisms involving calcium channels, and the role of T-type calcium channel Cav3.1 expression in this process is not well understood.
May support calcium blockade in experimental SAH; leaves open Cav3.1 role and clinical translation.
To study delayed cerebral vasospasm (DCVS) induced by subarachnoid hemorrhage (SAH), 60 healthy Sprague Dawley (SD) rats were randomly divided into 5 groups (12 rats in each group), namely sham operation group, blood injection model group, nimodipine group, flunarizine hydrochloride group, and normal group. Then, the physiological parameters were detected, and after the rats were killed under anesthesia, the degree of nerve injury, vasospasm as well as the therapeutic effect of drugs were evaluated by Western Blot (WB). Neurological impairment (NI), endothelial contraction and spasm were obvious in rats following blood injection. The expression of Cav3.1 on T-type calcium channels was significantly higher in the blood injection model group than in the sham operation group along with the normal group. Moreover, Cav3.1 mRNA was expressed in all groups. The Cav3.1 expression in blood injection model group and two drug groups were significantly higher than that in sham operation group and lower than that in blood injection model group. Vasospasm was improved in two drug groups, which indicated that calcium channel antagonists nimodipine and flunarizine hydrochloride had a certain therapeutic effect on DCVS in rats. The decrease in body weight and food intake of the two groups of rats treated with drugs decreased, and the delayed vasospasm was improved, but the expression of Cav3.1 was not changed significantly, indicating nimodipine and flunarizine hydrochloride had a therapeutic effect on delayed vasospasm in rats, but Cav3.1 expression on calcium channels was not affected.
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Zhang et al. (2020) studied Subarachnoid hemorrhage and delayed cerebral vasospasm (n=60). Nimodipine and Flunarizine hydrochloride vs. Sham operation and untreated blood injection model was evaluated on Cav3.1 expression on T-type calcium channels. Nimodipine and flunarizine hydrochloride improved delayed cerebral vasospasm and neurobehavioral scores in subarachnoid hemorrhage rats, but did not significantly alter Cav3.1 expression.
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