A method for rapid determination of human myoglobin was developed. The method is based on the technique of membrane immunoassay with light-sensitive nylon membranes for covalent immobilization of antibodies. The kinetic of binding of myoglobin with immobilized antibodies was studied. The pumping of reagents through the pores of a membrane allows to reduce the total assay time to 40 min. The sensitivity of membrane assay of myoglobin was 20 ng/ml. A semi-quantitative method with visual detection also was proposed. It allows to distinguish various states of myocardial infarction pathology.
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Rubtsova et al. (1994) studied this question.
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