ANALYSIS OF PHYTOCHEMICALS IN Holarrhena floribunda (G.Don) PARTS FOR ORGANIC MANURE PRODUCTION

The quest for sustainable agriculture has called for the use of plant based organic manure. This study examined the quality and quantity of phytochemicals components present in the leaf, stem-bark, and root of Holarrhena floribunda. Fresh leaf, stem-bark, and root of Holarrhena floribunda were collected separately in zip lock bags and well labelled. The samples were authenticated, rinsed with water, air dried under shade, and pulverized using a laboratory mill. Regular phytochemical screening procedures were carried out for qualitative analysis, while spectrophotometric techniques were used for quantitative analysis. Extractions and phytochemical analysis of H.floribunda leaf, stem-bark, and root were done using standard laboratory procedures. The results showed the presence of varied concentrations of, tannins, quinones, phenol, terpenoids, saponins, anthraquinone, coumarin, alkaloids, phytate, flavonoids and oxalate in all the plant parts been investigated. Abundant presence (+++) of alkaloids with a quantity of 0.205mg/g, demonstrating that the leaves are rich in nitrogen- containing secondary metabolites. Moderate concentration of tannins (10.000%), terpenoids (5.567%), quinones (1.820%), phytate (5.835%), phenol (2.555%), and oxalate (3.061%) were also recorded in the leaf of H. floribunda. The highest alkaloids concentration (0.245%) among the three plant parts, with strong qualitative presence (+++). This finding suggests that the root of H. floribunda serves as vital reservoir of bioactive alkaloids. This study concluded that the presence of phytochemicals both quantitatively and qualitatively in H. Floribunda is an indication that the plant parts can be processed for organic manual to enhance agricultural production.