Expression of Toxoplasma Gondii Recombinant Protein and Its Application in ELISA Detection

Toxoplasma gondii is a prevalent parasite with global distribution capable of infection various warm-blooded animals, potentially causing clinical manifestations such as dirrhea and miscarriage. Investigation its protein expression through exerimental approached can enhance scientific understanding of this pathogen. This study mainly focuses on the epidemiological samples of T. gondii using the enzyme-linked immunosorbent assay (ELISA) to detect protein expression. Experimental procedures included PCR amplification. protein induction, expression, and purification, with recombinant protein validation performed through protein elextrophoresis. Results demonstrated that among 60 tested samples, a high proportion of false positives was observed, which did not correlate with the expected positivity rate (4 confirmed cases). This discrepancy may stem from sample contamination during non-standardized experimental procedures. These findings provide a reference for future T. gondii research and contribute to vaccine development efforts against this parasite.