Spatially Progressive Penetration Self‐Propelled Nanomotors for Stepwise Eradication of Biofilm and Deep‐Tissue‐Infected Wounds Remodeling

Current therapies for chronic infected wounds primarily rely on multifunctional nanoplatforms with antibacterial, anti‐biofilm, and anti‐inflammatory properties. However, biofilms are often overlooked as the initial physical barrier that must be disrupted before therapeutic agents can effectively penetrate deep inflammatory tissues and eliminate residual infection. In addition, heterogeneous infection microenvironments demand versatile therapeutic systems. To address these challenges, this study developed self‐propelled nanomotors (Dex@CQDs@mPDA) and proposed a spatially progressive penetration strategy to achieve precise, stepwise treatment from the surface to deep tissues. First, dextran (Dex) surface modification enables targeted recognition of bacterial biofilms, while the near‐infrared (NIR) photothermal effect facilitates biofilm disruption. Subsequently, mesoporous polydopamine (mPDA) catalyzes H 2 O 2 to generate O 2 , which, together with carbon quantum dots (CQDs)‐mediated NO release, produces a dual‐gas‐driven propulsion effect, promoting active penetration into deep inflammatory tissues. Meanwhile, the nanomotors effectively scavenge excessive reactive oxygen species (ROS), promote macrophage polarization toward the M2 phenotype, enhance angiogenesis and collagen deposition, and remodel the inflammatory microenvironment to accelerate tissue regeneration. In vitro experiments further confirmed their excellent antibacterial, antibiofilm, and anti‐inflammatory activities, as well as their broad applicability across diverse infection scenarios. This work provides a therapeutic strategy that links biofilm eradication with deep inflammation resolution.