Why the study?
A simple and direct enzymic assay for measuring triglycerides in whole serum and lipoprotein fractions was needed to evaluate assay performance and accuracy.
A simple two-step procedure is now available for directly measuring triglycerides in whole serum and in lipoprotein fractions. I tested the performance of the assay, using pooled serum stored frozen, freshly prepared lipoprotein fractions, and pure glyceride standards. A parabolic concentration-response curve ensured linearity well beyond total glycerol concentrations of 10 mmol/L, but gave rise to misleading results for massively lipemic samples. Within-day CVs averaged 2.2% for the stored-frozen serum pools; mean day-to-day variation was 2.4%. Analytical recoveries of triglycerides, after lipoprotein fractionation, ranged between 101.9 and 103.7%. Aqueous glyceride standards gave results equivalent to between 95 and 105% of their glycerol content.
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Martin Kohlmeier (1986) studied Growth performance and lipid metabolism (n=600). Low dietary fish meal vs. High dietary fish meal (20% or 25%) was evaluated on Percent weight gain and survival (Weight gain decreased by 26.55% and survival decreased by 46.09% (5% vs 25% fish meal), p=<0.05).
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