What question did this study set out to answer?

To develop a fluorimetric micro-method for estimating glycerol in aqueous solution and apply it to determine triglyceride concentrations in serum lipid extracts.

January 1, 1961Journal of Lipid ResearchOpen Access

A fluorimetric micro glycerol method and its application to the determination of serum triglycerides

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Authors

DMDennis MendelsohnUniversity of the Witwatersrand AAA. AntonisBoston University

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Implication

Methodological study demonstrates a sensitive fluorimetric microassay for glycerol in serum extracts, highlighting its application for measuring triglycerides.

Key Points

To develop a fluorimetric micro-method for estimating glycerol in aqueous solution and apply it to determine triglyceride concentrations in serum lipid extracts.
Glycerol is heated with o-aminophenol in the presence of concentrated sulfuric acid and an oxidizing agent to form 8-hydroxyquinoline.
Fluorescence is induced and measured by chelating the synthesized 8-hydroxyquinoline with a divalent metal ion in an alkaline medium.
The chemical procedure was adapted to analyze phospholipid-free serum lipid extracts and phosphatide glycerol.
The chemical reaction sequence successfully produces measurable fluorescence proportional to glycerol content in aqueous solutions.
The fluorimetric technique reliably measures triglyceride concentrations in phospholipid-free serum extracts and enables the estimation of phosphatide glycerol.

Cite This Study

Mendelsohn et al. (1961) studied this question.

synapsesocial.com/papers/6a6fca3c26a7f98052dc63a9 https://doi.org/10.1016/s0022-2275(20)39037-4

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Implication

Methodological study demonstrates a sensitive fluorimetric microassay for glycerol in serum extracts, highlighting its application for measuring triglycerides.

A fluorimetric micro glycerol method and its application to the determination of serum triglycerides

Discussion

Member takes

Implication

Key Points

Cite This Study

A fluorimetric micro glycerol method and its application to the determination of serum triglycerides

Discussion

Member takes

Implication

Key Points

Cite This Study

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