Detection of Herpes Simplex Virus DNA in Cerebrospinal Fluid of Saudi Pediatric Patients with Encephalitis by the Polymerase Chain Reaction

Twelve samples of cerebrospinal fluid (CSF) from Saudi pediatric patients with suspected encephalitis were found negative for herpes simplex virus by cell culture and by nucleic acid hybridization using(32)P-labeled specific probes. After amplification of DNA extracted from 100 microl of each sample using primers for the glycoprotein D gene of HSV, seven specimens were found positive for HSV DNA. Subsequent hybridization with the labeled DNA probe ensured the positivity of the seven samples and further detected one more positive sample, for a total of eight HSV DNA positive cases. This technique improved the detection of HSV in CSF samples and may assist in an early diagnosis for HSV ecephalitis.