Key result
Pulmonary artery banding induces rapid, sustained RV remodeling and dysfunction in mice by ~3 days.
Why the study?
Right ventricular failure is the leading cause of death in pulmonary hypertension, and a reliable mouse model of pressure-overload-induced RV failure is needed for research.
This protocol provides a detailed, reproducible surgical method for generating a pressure-overload right ventricular failure model in mice with low intraoperative mortality.
Right ventricular failure (RVF) is the leading cause of death in patients with pulmonary hypertension. Here, we present a protocol for pulmonary artery banding in mice to generate a model of pressure-overload-induced RVF. We describe steps for anesthesia of mice, endotracheal intubation, and pulmonary artery banding surgery. We then detail procedures for phenotyping and analysis. Our approach does not involve complete blockage of the pulmonary flow during clip placement and is, therefore, associated with low intraoperative mortality. For complete details on the use and execution of this protocol, please refer to Veith et al. (2020).1
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Mamazhakypov et al. (2023) studied Right ventricular failure. Pulmonary artery banding vs. Sham operation was evaluated on Right ventricular remodeling and dysfunction (RV function, geometry, and hemodynamics) (p=<0.05). Pulmonary artery banding in mice induced significant right ventricular remodeling and dysfunction as early as 3 days post-surgery, sustained for 5 weeks.
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