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An automated fluorometric microtechnic, suitable for screening purposes, has been developed for the quantitative determination of glucose-6-phosphate dehydrogenase (G6PD) and 6-phosphogluconate dehydrogenase (6PGD) levels in red blood cells. The method is rapid, simple, and precise. It uses 50 µl whole-blood (capillary) samples and is based on the measurement of natural fluorescence emitted by the NADPH generated during the enzymatic reactions.
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Tan et al. (1969) studied this question.
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