Key result
Optimized single-thoracotomy MIRI model matches traditional lesion sizes while improving survival and cutting surgical time.
Why the study?
Conventional mouse models of myocardial ischemia-reperfusion injury rely on indwelling support tubes and secondary thoracotomy for reperfusion, resulting in complicated operations, high pneumothorax risk, severe thoracic trauma, and low batch modeling efficiency.
Does an optimized single-thoracotomy MIRI mouse model using carbon-synthesis fishing line pull-out improve modeling efficiency, safety, and reproducibility compared to traditional secondary-thoracotomy modeling in male C57BL/6 mice?
Does an optimized single-thoracotomy MIRI mouse model using carbon-synthesis fishing line pull-out improve modeling efficiency, safety, and reproducibility compared to traditional secondary-thoracotomy modeling in male C57BL/6 mice?
An optimized mouse model of myocardial ischemia-reperfusion injury utilizing a single thoracotomy and carbon-synthesis fishing line pull-out produces equivalent ischemic lesions to traditional models while improving animal survival, surgical efficiency, and reproducibility.
Does not inform clinical MIRI management; extends options for efficient, reproducible mouse models.
Myocardial ischemia-reperfusion injury (MIRI) severely impairs cardiac prognosis after revascularization for acute myocardial infarction. Standardized mouse left anterior descending coronary artery (LAD) ligation models are widely used for MIRI research, yet conventional protocols rely on indwelling support tubes and secondary thoracotomy to restore reperfusion. Such procedures feature complicated operations, high risks of pneumothorax, severe thoracic trauma, and low batch modeling efficiency. To address these drawbacks, we established an optimized MIRI mouse model with a single thoracotomy and carbon-synthesis fishing line pull-out for reperfusion. Male C57BL/6 mice aged 6-8 weeks were divided into normal control (NC), traditional model (TM), and optimized model (OM) groups. Echocardiography, triphenyltetrazolium chloride (TTC) and hematoxylin-eosin (HE) staining, as well as serum inflammatory cytokines, lactate dehydrogenase (LDH), c-reactive protein (CRP), and myocardial injury marker cardiac troponin I (cTnI) levels were detected to compare modeling efficacy. Identical cardiac function, serum inflammatory cytokines, myocardial injury marker, infarct size, and myocardial pathological morphology changes were observed between TM and OM groups. The OM group displayed more homogeneous infarct regions and higher serum myocardial injury markers and cardiac function injury, indicating milder non-surgical trauma. Compared with the traditional method, this optimized procedure shortens surgical and anesthesia time, avoids fatal intraoperative pneumothorax, accelerates post-operative recovery, and improves animal survival. In conclusion, the optimized model produces equivalent MIRI lesions with higher safety, efficiency, and reproducibility. It serves as an ideal alternative to classic modeling approaches.
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Jiang et al. (2026) studied Myocardial ischemia-reperfusion injury (MIRI). Optimized MIRI mouse model (single thoracotomy and carbon-synthesis fishing line pull-out for reperfusion) vs. Traditional MIRI model (indwelling support tubes and secondary thoracotomy) and normal control was evaluated on Cardiac function, infarct size, myocardial pathological morphology, serum inflammatory cytokines, LDH, CRP, and cTnI levels. An optimized single-thoracotomy mouse MIRI model produced equivalent myocardial lesions to the traditional model while shortening surgical time, avoiding pneumothorax, and improving survival.
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