Uji Aktivitas Antihipertensi Ekstrak Daun Ulin (Eusideroxylon zwageri) pada Tikus Putih (Rattus norvegicus) dengan Metode Noninvasif

Hypertension is a major health problem associated with high morbidity and mortality, prompting the exploration of natural products as potential sources of antihypertensive agents. Ulin (Eusideroxylon zwageri) is a plant native to Kalimantan that contains flavonoids with antioxidant properties. However, its antihypertensive potential has not been investigated in vivo using a prednisone NaCl induced hypertension model with non-invasive blood pressure measurement. This study aimed to identify the antihypertensive effectiveness of ulin leaf extract in reducing systolic and diastolic blood pressure in Wistar rats induced with prednisone and NaCl. A true experimental study with a pretest-posttest control group design was conducted using 25 male Wistar rats allocated into five groups, negative control (0.5% NaCMC), positive control (captopril, 2.25 mg/kgBW), and ulin leaf extract at doses of 10, 25, and 50 mg/kgBW. The extract was prepared by maceration and subjected to phytochemical screening. The study lasted 21 days, comprising 14 days of hypertension induction and 7 days of treatment. Blood pressure was measured using a non-invasive method, and the data were analyzed using ANOVA. Phytochemical screening revealed the presence of flavonoids, tannins, saponins, alkaloids, steroids, and terpenoids. Administration of ulin leaf extract at doses of 10, 25, and 50 mg/kgBW reduced systolic and diastolic blood pressure (p < 0.05). Ulin leaf extract demonstrated antihypertensive activity in prednisone NaCl induced Wistar rats, as indicated by its ability to reduce systolic and diastolic blood pressure.