D3.1. Detailed protocols for each liquid biopsy pilot study

The primary objective is to define, collect, and harmonize standard operational protocols for the 7 Liquid Biopsy Pilot Studies planned in SPARC. These pilot studies span multiple oncology indications, including more than 11 cancers. Moreover, they are designed to drive the real-world clinical implementation of liquid biopsy across European healthcare systems.To achieve technical rigor and inter-centre comparability across heterogeneous European healthcare settings, WP3 executed a structured operational strategy. This included a baseline survey (M2–M4), a centralized Excel Pilot Monitoring Tool (M8) for real-time tracking of the pilot progress, and a standardized Protocol Template (M9). Together, these tools establish minimum requirements across pre-analytical, analytical, and clinical workflows, directly supporting the progression of each pilot along the Translation Level of Study (TLS) scale.