Optimization of the Technology of Suppositories with CO2 Extract of Rosmarinus officinalis L. and In Vitro Evaluation of Chamazulene Release

This study investigated manufacturing conditions for suppositories containing a CO2 extract of Rosmarinus officinalis L. herb, using a previously developed formulation, and evaluated a gas chromatography procedure with flame ionization detection (GC–FID) for quantitative marker determination. The effects of key manufacturing parameters were evaluated, and the optimal conditions for base preparation were determined as 70 °C and 120 rpm for 12 min; increasing the agitation speed to 150 rpm did not reduce the processing time. Extract concentrate preparation at 37 °C and 60 rpm required 25 min to achieve visually assessed homogeneity. At 60 rpm, prior concentrate preparation reduced the subsequent mixing time of the suppository mass from 100 to 20 min at each investigated temperature (37, 45, and 60 °C), excluding the time required to prepare the concentrate. Cooling at 8 °C for 60 min was selected on the basis of mechanical-strength measurements. Agar diffusion experiments provided a comparative assessment of the diffusion of colored extract constituents rather than a quantitative measure of chamazulene release or bioavailability. The findings identify candidate manufacturing conditions for further evaluation. Confirmation of marker identity, analytical validity, quantitative content uniformity, and long-term stability is required before reliable product standardization can be established.