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Adeno-associated vector is a platform of choice for delivering genome-editing toolkits, due to its transient and robust expression. Here, we present a protocol for viral production for in vivo use. We describe steps for transfecting producer cells, harvesting virus, and purifying/concentrating viral particles. We then detail procedures for estimating viral titers. This protocol ensures high viral yields and transduction competency and is scalable to meet user-customized production needs. For complete details on the use of this protocol, please refer to Kantor et al. 1 1. Kantor, B. ∙ O'Donovan, B. ∙ Rittiner, J. ... The therapeutic implications of all-in-one AAV-delivered epigenome-editing platform in neurodegenerative disorders Nat. Commun. 2024; 15 :7259 Crossref Scopus (38) PubMed Google Scholar
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Duke et al. (2026) studied this question.
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