Taxonomic descriptions of Tessaracoccus zhenghani sp. nov., Tessaracoccus pujii sp. nov., Tessaracoccus luoxuelianae sp. nov. and Tessaracoccus yangjingae sp. nov.

Eight Gram-stain-positive, non-spore-forming, non-motile, oxidase-negative novel bacterial strains with oval- to rod-shaped morphology designated Y36ᵀ, Y1639, Z1128ᵀ, Y1544, Y1736ᵀ, W1776, G1721ᵀ and Y1940 were isolated from the in situ coal and coal mine dust matrices in Shanxi Province, PR China. Phylogenetic analysis of the 16S rRNA gene and 813 core genes revealed that these 8 strains belong to the genus Tessaracoccus . 16S rRNA gene sequence analysis revealed that strain Y36 T exhibited the highest similarity to Tessaracoccus lubricantis KSS-17Se T (98.42%), while strains Z1128 T , Y1736 T and G1721 T showed the highest similarities to Tessaracoccus aquimaris NSG39 T (98.61%, 98.47% and 97.64%, respectively). Average nucleotide identity (ANI) and digital DNA–DNA hybridization (dDDH) values between these four novel species and all members of the genus Tessaracoccus were below species thresholds (95–96% for ANI and 70% for dDDH). Anteiso-C 15:0 was the predominant cellular fatty acid in all eight isolates. Summed feature 3 (C 16:1 ω 7c/C 16:1 ω 6 c ) was also a major component in strains Z1128ᵀ, Y1544, Y1736ᵀ, W1776, G1721ᵀ and Y1940. Strain Y1736ᵀ additionally contained anteiso-C 17:1 ω 9c as a major fatty acid. All novel type strains had MK-9 (H4) as the sole respiratory quinone. The major polar lipids of strains Y36ᵀ and Y1736ᵀ were diphosphatidylglycerol and phosphatidylglycerol. Strains Z1128ᵀ and G1721ᵀ contained diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol and phosphatidylinositol mannoside as their major polar lipids. Strains Y36ᵀ and G1721ᵀ contained ribose as the sole cell-wall sugar. Strain Z1128ᵀ had ribose and glucose, while strain Y1736ᵀ harboured rhamnose, ribose and glucose as the major cell-wall sugars. The cell-wall peptidoglycan contained ʟʟ-diaminopimelic acid as the diagnostic diamino acid. Based on genotypic, phenotypic, phylogenetic and biochemical properties, the eight uncharacterized strains represent four novel species in the genus Tessaracoccus , for which the names Tessaracoccus zhenghani sp. nov. (type Y36 T =GDMCC 1.5638 T =KCTC 59558 T ), Tessaracoccus pujii sp. nov. (type strain Z1128 T =GDMCC 1.5727 T =KCTC 59595 T ), Tessaracoccus luoxuelianae sp. nov. (type strain Y1736 T =GDMCC 1.5728 T =KCTC 59596 T ) and Tessaracoccus yangjingae sp. nov. (type strain G1721 T =GDMCC 1.5729 T =KCTC 59594 T ) are proposed, respectively.