Protocol for aggregate and cumulative population cancer risk assessment of environmental pesticides

Environmental pesticide residues persist across air, soil, and water, causing long-term human exposure through ingestion, inhalation, and dermal contact. Here, we present a protocol assessing aggregate and cumulative population cancer risk of environmental pesticide mixtures using an integrated framework. We describe steps for compiling structured environmental pesticide monitoring datasets, regionalizing population exposure, and estimating aggregate exposure doses. We then detail procedures for calculating and mapping cumulative population cancer risk. This protocol supports risk comparison, hotspot identification, and science-based risk management. For complete details on the use and execution of this protocol, please refer to Huang and Li. 1