An in vitro study on thrombolytic action of Bothrops lanceolatu s in 6C and 30C potencies

Objectives: To evaluate the in vitro thrombolytic activity of Bothrops lanceolatus 6C and 30C using a standardised clot-lysis assay. Materials and Methods: Blood samples obtained from ten healthy volunteers were used for the in vitro clot-lysis assay. Blood clots were prepared in pre-weighed microcentrifuge tubes and exposed to Bothrops lanceolatus 6C and 30C. Distilled water and streptokinase served as the negative and positive controls, respectively. Following incubation, thrombolytic activity was assessed by measuring the reduction in clot weight. Results: Both potencies of Bothrops lanceolatus demonstrated in vitro clot-lytic activity, with the 6C potency showing greater consistency in clot dissolution than the 30C potency. Streptokinase produced the greatest degree of clot lysis, whereas distilled water showed negligible activity. Statistical analysis demonstrated a significant reduction in clot weight across the treatment groups ( p < 0.05). Conclusion: Under the experimental conditions of this study, Bothrops lanceolatus demonstrated measurable in vitro thrombolytic activity, with 6C showing a more consistent effect than 30C. These preliminary findings warrant further investigation using adequately powered experimental studies to characterise the observed activity and determine its reproducibility and potential clinical relevance.