Abstract 1450: 3D holotomography-enabled virtual multiplexed staining of thick medical kidney tissue for comprehensive renal pathology.

Abstract Renal pathology relies on multiple special stains—including H0.85. Virtual stains restored key elements—including basement membranes, mesangial matrix, tubular epithelium, and interstitial collagen—closely matching chemical references. Large-region predictions preserved coherent architecture. Virtual PAS and AFOG highlighted basement membranes, glycogen-rich areas, collagen deposition, and casts, supporting interpretation of glomerular injury and interstitial fibrosis. Robust performance was maintained in thick tissues (10–50 μm), where chemical stains often show uneven penetration; virtual stains preserved uniform contrast and delineated overlapping structures. Multiplexing enabled consistent visualization of basement membrane thickening, mesangial expansion, and inflammation within the same section, removing serial-section artifacts.Holotomography with generative translation enables reliable, label-free virtual multiplexed staining, producing H Part 1 (Regular Abstracts); 2026 Apr 17-22; San Diego, CA. Philadelphia (PA): AACR; Cancer Res 2026;86(7 Suppl):Abstract nr 1450.