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February 20, 2026Journal of Pineal Research0 citations

EXPRESSION OF CONCERN: Melatonin Protects Against Isoproterenol‐Induced Alterations in Cardiac Mitochondrial Energy‐Metabolizing Enzymes, Apoptotic Proteins, and Assists in Complete Recovery From Myocardial Injury in Rats

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Key Result

Melatonin protected rats from isoproterenol-induced cardiac mitochondrial enzyme and apoptotic protein alterations and aided complete myocardial injury recovery.

Key Points

  • The aim is to highlight concerns regarding data integrity in a study examining melatonin's protective effects on cardiac injury.
  • Review of data from the original study published in 2012.
  • Investigation into the potential reuse of beta-actin control bands.
  • Response from authors regarding the study's experimental design.
  • Concerns raised about duplicate beta-actin control bands.
  • Authors claimed studies were part of the same experimental project.
  • Original data validation not possible due to loss of samples.

Structured PICO

P
Population
Rats with isoproterenol-induced myocardial injury
I
Intervention
Melatonin
O
Outcome
Alterations in cardiac mitochondrial energy-metabolizing enzymes, apoptotic proteins, and recovery from myocardial injurysurrogate

This Expression of Concern alerts readers to potential data integrity issues regarding duplicated western blot control bands in a 2012 rat study on melatonin and myocardial injury.

Limitations

  • Original data no longer available to validate concerns
  • Duplicated beta-actin control bands
  • Failure to declare re-use of control bands from an earlier article

Abstract

EXPRESSION OF CONCERN : D. Mukherjee, A. K. Ghosh, A. Bandyopadhyay, et al., “Melatonin Protects Against Isoproterenol‐Induced Alterations in Cardiac Mitochondrial Energy‐Metabolizing Enzymes, Apoptotic Proteins, and Assists in Complete Recovery From Myocardial Injury in Rats,” Journal of Pineal Research 53, no. 2 (2012): 166–179, https://doi.org/10.1111/j.1600-079X.2012.00984.x . This Expression of Concern is for the above article, published online on 20 February 2012 in Wiley Online Library ( wileyonlinelibrary.com ), and has been issued by agreement between the journal Editor‐in‐Chief, Gianluca Tosini; and John Wiley & Sons Ltd. A third party raised concerns that the beta‐actin control bands in Figures 3A, 4A, 4B, 5A, 5B, 6A, 6B, 7A, and 7B were duplicates and had also been used in an earlier article by most of the same authors Mukherjee et al. 2010 ( https://doi.org/10.1111/j.1600-079X.2010.00749.x ). The authors responded to an inquiry by the publisher and stated that the studies reported in both articles were part of a single experimental project conducted between 2008 and 2009, which used identical sample sets processed under uniform conditions. Most of the original data were no longer available. As such, the publisher is not able to validate the data related to these concerns. Additionally, this article did not adequately indicate that both articles derived from the same experimental procedure and did not declare the re‐use of beta‐actin control bands. This Expression of Concern has been published in order to inform and alert readers about these concerns. The authors were informed of this Expression of Concern.

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Cite This Study

A 2026 study studied this question. Melatonin protected rats from isoproterenol-induced cardiac mitochondrial enzyme and apoptotic protein alterations and aided complete myocardial injury recovery.

synapsesocial.com/papers/6997fa35ad1d9b11b3453460https://doi.org/10.1111/jpi.70125
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Also Consider

Synapse has enriched 5 closely related papers on similar clinical questions. Consider them for comparative context:

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