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November 1, 2001Experimental PhysiologyOpen Access

Functional properties and responses to ischaemia‐reperfusion in Langendorff perfused mouse heart

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Population

Langendorff perfused C57/BL6 mouse hearts

Comparison

Isovolumic ventricular balloon model with 10-30… vs Apicobasal displacement model

Design

Preclinical

Follow-up

40 min reperfusion

Key result

The buffer perfused isovolumically contracting mouse heart model is more reliable and yields higher post-ischaemic recoveries than the apicobasal model.

Authors

JHJohn P. HeadrickJPJason N. PeartBHBen Hack

Discussion

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Overview

The isovolumic model offers superior reliability for mouse ischemia experiments; challenges apicobasal use and leaves open human translation.

Structured PICO

P
Population
C57/BL6 mouse hearts subjected to 10-30 min global ischaemia to assess functional properties and responses to ischaemia-reperfusion.
I
Intervention
Isovolumic ventricular balloon model with 10-30 min global ischemia and 40 min reperfusion
C
Comparator
Apicobasal displacement model
O
Outcome
Post-ischemic recovery of developed pressure and contractility (+dP/dt)surrogate

Main Result

Absolute Event Rate: 82% vs 80%

The buffer perfused isovolumically contracting mouse heart model is more reliable in assessing ischemic responses than the commonly employed apicobasal model.

Cite This Study

Headrick et al. (2001) studied Myocardial ischaemia and reperfusion. Isovolumic ventricular balloon model vs. Apicobasal displacement model was evaluated on Post-ischaemic recovery of developed pressure after 10 min ischaemia. The buffer perfused isovolumically contracting mouse heart model is more reliable and yields higher post-ischaemic recoveries than the apicobasal model.

synapsesocial.com/papers/6a22076e2221fd35d14929cchttps://doi.org/10.1111/j.1469-445x.2001.tb00035.x
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Also Consider

Synapse has enriched 5 closely related papers on similar clinical questions. Consider them for comparative context:

  1. 1Relationship between ischaemic time and ischaemia/reperfusion injury in isolated Langendorff‐perfused mouse hearts2001 · 30 citations
  2. 2Characterization of the Langendorff Perfused Isolated Mouse Heart Model of Global Ischemia–Reperfusion Injury2015 · 59 citations
  3. 3Measurement of coronary flow reserve in isolated hearts from mice2004 · 23 citations
  4. 4Cyclic fluctuations in the cardiac performance of the isolated Langendorff‐perfused mouse heart: pyruvate abolishes the fluctuations and has an anti‐ischaemic effect2002 · 16 citations
  5. 5Development and characterization of a mouse in vitro model of ischaemia-induced ventricular fibrillation2009 · 31 citations