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February 19, 2009Cardiovascular Research

Development and characterization of a mouse in vitro model of ischaemia-induced ventricular fibrillation

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Population

Hearts from male Tuck Ordinary mice

Comparison

Perfusion with Krebs solution containing… vs Krebs-perfused hearts without catecholamines…

Design

Preclinical

Follow-up

30 min regional ischaemia and 10 min reperfusion

Authors

CSCatherine L. StablesUniversity of OxfordMCMichael J. CurtisElectrophysiology

Discussion

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Implication

Should not alter clinical VF management; extends Langendorff models to enable functional genomic arrhythmia studies.

Structured PICO

P
Population
Hearts from male Tuck Ordinary mice
I
Intervention
Perfusion with Krebs solution (modified to contain low-normal K+ 3 mmol/L, and high Ca2+ 2.4 mmol/L) containing catecholamines (epinephrine 313 nmol/L plus norepinephrine 75 nmol/L) and/or angiotensin II (100 pmol/L), with or without supraventricular pacing (600 b.p.m.)
C
Comparator
Krebs-perfused hearts without catecholamines, angiotensin II, or pacing
O
Outcome
Incidence of ischaemia-induced and reperfusion-induced ventricular fibrillation (VF)surrogate

A novel mouse Langendorff model successfully evokes regional ischaemia-induced ventricular fibrillation using periphysiological perfusion conditions, enabling future functional genomic studies.

Cite This Study

Stables et al. (2009) studied this question.

synapsesocial.com/papers/6a6fbd9d87f9210571274bc0https://doi.org/10.1093/cvr/cvp068
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Also Consider

Synapse has enriched 5 closely related papers on similar clinical questions. Consider them for comparative context:

  1. 1Characterisation, utilisation and clinical relevance of isolated perfused heart models of ischaemia-induced ventricular fibrillation1998 · 120 citations
  2. 2Independent contribution of catecholamines to arrhythmogenesis during evolving infarction in the isolated rat heart2002 · 31 citations
  3. 3In Vivo Cardiac Electrophysiology Studies in the Mouse1996 · 229 citations
  4. 4Measurement of heart rate and Q-T interval in the conscious mouse1998 · 553 citations
  5. 5Influence of Tedisamil on the Initiation and Maintenance of Ventricular Fibrillation1991 · 66 citations