August 2, 2025Open Access

Brr2p-mediated unwinding of U4/U6 is promoted by a mutually exclusive intra-molecular stem loop in U4 and involves destabilization of the 5′ stem-loop of U4

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Screen reveals Brr2p's role in disrupting U4 snRNA structures to promote U4/U6 unwinding, indicating implications for spliceosome function.

Brr2p is essential for unwinding the U4/U6 snRNAs in spliceosome activation.
Destabilizing mutations in U4 suppress a brr2 mutant, highlighting U4's role in unwinding.
The U4-ISL1 stem loop is crucial for splicing and U4/U6 unwinding in vitro.
Findings suggest Brr2p disrupts all key U4 secondary structures during unwinding process.

Nielsen et al. (2025) studied this question.

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Screen reveals Brr2p's role in disrupting U4 snRNA structures to promote U4/U6 unwinding, indicating implications for spliceosome function.