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August 6, 2025Nucleic Acids ResearchOpen Access

ONE-STEP tagging: a versatile method for rapid site-specific integration by simultaneous reagent delivery

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Authors

VMValentina MiglioriWellcome Sanger InstituteMBMichaela BruntraegerWellcome Sanger InstituteIGIvan GyulevWellcome Sanger Institute

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Implication

Genome editing method ONE-STEP tagging achieves high integration efficiency in hiPSCs and T cells, indicating it may advance therapeutic applications.

Key Points

  • Integration efficiency reached about 90% at the ACTR10 locus in hiPSCs, showcasing the method's precision in genome editing.
  • Using a dual-cassette approach, ONE-STEP tagging enables multiplexed tagging and simultaneous editing at multiple loci.
  • Application in primary T cells demonstrated effective targeting of the T cell receptor locus while knocking out B2M, facilitating CAR T cell development.
  • The system enhances integration capabilities for large DNA constructs and supports various cell types, pointing to its therapeutic potential.

Cite This Study

Migliori et al. (2025) studied this question.

synapsesocial.com/papers/68af474ead7bf08b1ead3c55https://doi.org/10.1093/nar/gkaf809
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