This protocol assesses precopulatory behaviour changes in Parhyale hawaiensis due to phenanthrene exposure, highlighting potential reproductive disruptions.
This protocol describes a time-to-event assay designed to assess the impact of phenanthrene exposure on precopulatory behaviour in the tropical amphipod Parhyale hawaiensis. Pairs with a prior mating history are individually exposed to graded concentrations of phenanthrene and subsequently reintroduced to quantify latency to re-engage in amplexus. Using survival analysis, this method enables the detection of sublethal, behaviourally mediated effects of contaminant exposure on reproductive function. The approach is cost-effective, reproducible, and suitable for screening chemosensory disruption in small crustaceans.
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Ibrahim Lawan (2025) studied this question.
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