Assessing Fluoranthene Impact on Initiation of Feeding Response: A Time-to-Event Experiment with Parhyale hawaiensis v1

This protocol delineates a controlled laboratory experiment employing Parhyale hawaiensis, an amphipod crustacean, as a model organism to assess the influence of Fluoranthene, a polycyclic aromatic hydrocarbon (PAH), on the dynamic chemosensory responses of P. hawaiensis. Using a time-to-event (TTE) methodology, we examine how Fluoranthene exposure affects the timing of crucial behavioural responses essential for organism survival and reproduction. This protocol offers comprehensive step-by-step instructions for conducting TTE experiments, providing valuable insights into the behavioural ecotoxicological impacts of Fluoranthene on aquatic organisms.