Figure 1 from Plasma Proteomic Analysis Reveals Complement System Changes in Irradiated Female BALB/c Mice during Mammary Carcinogenesis

Experimental approach. A, The endogenous epithelium of the inguinal mammary glands was surgically removed from 3-week-old mice. These mice were then aged to 10 weeks, irradiated with 50 cGy of γ-ray, and transplanted with Trp53-null mammary fragments. Half of the mice received low-dose aspirin in their drinking water for 6 months. All mice were monitored by palpation, and subsets were euthanized at 4 and 8 months for plasma collection. The remaining mice were observed until tumor development or the study’s conclusion at 550 days after transplantation. Tissue samples were collected from mice that remained tumor-free up to 550 days of age. B, Graphical workflow of the proteomics. Asp, aspirin; Hp-RP, high pH reversed-phase.