December 12, 2024

Single Cell Multiomics protocol for processing gDNA and mRNA v1

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This protocol demonstrates single cell multiomics by extracting gDNA and cDNA, suggesting refined replication timing analysis in cell lines.

The multiomics protocol successfully extracts gDNA and cDNA from single cells, enhancing genomic analysis.
It employs SMARTSeq2 for mRNA processing, enabling detailed insights into copy number variation.
This approach supports replication timing analysis using computational pipelines compatible with gDNA protocols.
Accessibility of this protocol may encourage broader applications in single-cell genomics research.

Shetty et al. (2024) studied this question.

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This protocol demonstrates single cell multiomics by extracting gDNA and cDNA, suggesting refined replication timing analysis in cell lines.