Figure 2 from Dietary Fiber Lacks a Consistent Effect on Immune Checkpoint Blockade Efficacy Across Diverse Murine Tumor Models

Purified diet has a greater impact than fiber on the serum and fecal metabolome. A, PCA of serum metabolomics from the B16-OVA experiment. PC, principal component. B, Heatmap showing log-transformed relative abundances of top 50 significant metabolites by ANOVA of the same metabolomics dataset. C, Volcano plots of serum metabolites in mice fed with chow vs. low–fiber-purified diet (LC_In0) and in mice fed with high–fiber (HC_In8)- vs. low–fiber (LC_In0)-purified diet (all B16-OVA tumors and anti–PD-1 treatment). FC, fold change. D, Venn diagram of significantly changed metabolites from the comparisons shown in C, showing minimal overlap between the high–fiber-purified diet and chow. For all panels, n = 7 to 8 mice per group.