Phytochemical Profiling and Pharmacological Evaluation of the Methanol Extract of Persicaria chinensis L.H. Gross Leaves for Thrombolytic, Antidepressant, and Anxiolytic Activities through the In Vitro and In Vivo Approach

Objective: Persicaria chinensis L.H. Gross is an herbal member of the Persicaria genus and the Polygonaceae family. The objective of this study was to analyze the thrombolytic, antidepressant, and anxiolytic activities of the methanol extract of Persicaria chinensis leaves. Materials and Methods: The current study uses several different experimental methods to investigate the presence of bioactive metabolites and the roles that Persicaria chinensis methanol leaf extract (MEPCL) plays in reducing thrombosis, anxiety, and depression. Blood was withdrawn from volunteers who underwent an in vitro examination to assess thrombolytic activity. The anxiolytic effects were observed by employing elevated plus maze (EPM) and hole-board (HBT) tests, while the antidepressant effects were evaluated using forced swimming (FST) and tail suspension tests (TST). Results: Alkaloids, steroids, saponins, glycosides, phenols, tannins, flavonoids, terpenoids, quinones, and reduced sugar are the secondary metabolites present in MEPCL, which was the preliminary investigation of this study. The MEPCL showed mild thrombolytic activity. The immobility duration of both doses of MEPCL was significantly decreased in TST and FST. In HBT, the higher doses showed a considerable increase in head dipping, and, in the EPM test, time spent in the open arm was increased significantly with an increased dose. Conclusion: This herb has potential for managing thrombosis, anxiety, and depression-like disorders.