In Vivo Imaging of Xenon Biosensors using Hyperpolarized 129Xe MRI

Motivation: Molecular MRI based on hyperpolarized xenon biosensors is a promising approach for detecting biological processes due to its superior signal and chemical shift sensitivity. However, in vivo applications remain challenging. Goal(s): To investigate the distribution and dynamics of xenon biosensors in vivo. Approach: A robust method was developed for direct imaging of 129Xe in cage molecules. The decay time constant (Tdecay) and exchange time constant (Tex) of the caged 129Xe were measured in vivo. Results: Caged 129Xe was accurately imaged. Moreover, significant differences were found in Tdecay across different brain regions and in Tex between glioma-bearing rats and controls. Impact: Our findings advance molecular MRI technology by improving understanding of xenon biosensor distribution and dynamics. This approach lays the groundwork for future clinical applications of hyperpolarized xenon biosensors.