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September 23, 2025BMC Infectious DiseasesOpen Access

Comparative DNA extraction methods to use for LAMP assay as molecular diagnosis of human schistosomes from urine samples

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Authors

BPBrittany R. PulkkilaCSChummy SikasungeJMJames Mwansa

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Overview

Comparison of extraction techniques reveals varying sensitivity and cost-effectiveness for schistosomiasis diagnosis.

Key Points

  • Qiagen extraction method detected positive schistosomiasis infections and was more cost-effective than LAMP-PURE.
  • LAMP assay with LP extraction was the fastest at 20 minutes, but incurred higher costs compared to other methods.
  • Chelex extraction identified 20% more positive infections than heating extraction, despite being slower.
  • Heating extraction was fast and inexpensive but led to false negatives during LAMP amplification.

Cite This Study

Pulkkila et al. (2025) studied this question.

synapsesocial.com/papers/68d4724731b076d99fa6aa26https://doi.org/10.1186/s12879-025-11370-y
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