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June 13, 2026Tropical Medicine and Infectious DiseaseOpen Access

Rapid and Sensitive Detection of Schistosoma mansoni in the Intermediate Snail Hosts Using Loop-Mediated Isothermal Amplification (LAMP) Diagnostics

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Authors

HLHongmei LiZQZhiqiang QinSLShan Lv

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Overview

Randomized trial demonstrates sensitive detection of Schistosoma mansoni in snails, indicating improved surveillance methods.

Key Points

  • This research aims to develop a sensitive diagnostic assay for detecting Schistosoma mansoni in intermediate snail hosts.
  • Developed smND1-LAMP assay targeting ND1 gene at 65 °C for 1 hour.
  • Evaluated diagnostic performance using laboratory-infected Biomphalaria snails and field samples from Zimbabwe and Burkina Faso.
  • Compared smND1-LAMP with microscopy, conventional PCR, and SYBR Green real-time PCR.
  • smND1-LAMP achieved 100% specificity and 75% sensitivity in laboratory tests.
  • In field surveys, smND1-LAMP detected 25.9% positive snails versus 22.2% with conventional PCR.
  • Microscopy failed to detect any positive snails, highlighting smND1-LAMP's effectiveness.

Cite This Study

Li et al. (2026) studied this question.

synapsesocial.com/papers/6a2cf604faef96ed7f057d29https://doi.org/10.3390/tropicalmed11060157
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Also Consider

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  1. 1Test accuracy of loop-mediated isothermal amplification for schistosomiasis in low endemicity areas: a systematic review and meta-analysis2025 · 7 citations
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