Exploration of a Traditional Nanomedicine - Mukta Pishti Subjected to Bhavana with Gulaba Arka for 3, 7, and 21 Days

Introduction: Mukta pishti , a traditional Ayurvedic formulation, has been extensively used for its therapeutic benefits. Pearls are available in natural and cultured forms; however, the scarcity and price have limited the use of natural pearls in medicine. Thus, cultured pearls were used for this study. This study aims to characterize the pharmaceutico-analytical characteristics of Mukta pishti given 3, 7, and 21 days Bhavana , using various modern analytical techniques to determine its quality, purity, and therapeutic potential. Materials and Methods: Mukta pishti was prepared as mentioned in the Ayurvedic texts. Physicochemical and instrumental analysis was done before and after the procedure. Results: This study presents the detailed findings on composition, crystalline structure, surface morphology, particle size, and stability. X-ray diffraction patterns of Mukta pishti showed distinct peaks corresponding to calcium carbonate in the form of aragonite. Scanning electron microscope analysis shown micro fineness of the particle size. Inductively Coupled Plasma Atomic Emmision Spectroscopy (ICPAES) confirmed the presence of calcium carbonate as the primary component, along with trace elements such as magnesium, iron, manganese, zinc, etc. Fourier transform infrared spectra indicated the presence of characteristic functional groups corresponding to calcium carbonate and other organic compounds. Zeta Potential analysis showed a high level of stability in all three samples, indicating strong repulsive forces between particles, preventing aggregation. Conclusion: The comprehensive evaluations ensure, Mukta pishti prepared with Gulab arka has enhanced nano particulate formation, consistent elemental composition, and enhanced stability. These findings imply higher bioavailability and therapeutic effectiveness, validating the traditional use of Gulab Arka in Mukta pishti preparation.