Figure 2 from Preclinical Evaluation of Folate Receptor-α Chimeric Antigen Receptor T Cells Exhibits Highly Efficient Antitumor Activity against Osteosarcoma

FH FOLR1-CART antitumor activity against U-2 OS osteosarcoma cell line in vitro. A, FOLR1 expression detected by flow cytometry by U-2 OS, WSU-AML (standard M7, FOLR1-positive AML), and Kasumi (standard FOLR-negative AML). Red, tumor stained with PE-labeled anti-FOLR1; unfilled, isotype control. B, Increased FH FOLR1-CAR T cell–induced tumor cell lysis detected by flow cytometry after co-culture at 6 and 24 hours at various E:T ratios, compared with tumor cell lysis induced by NT T cells. C, Representative images from the IncuCyte imaging platform at hours 0 and 20.5 of co-culture. Green, live U-2 OS tumor cells; red, dead cells. D, Concentration (pg/mL) of secreted IL-2, IFN-γ, and TNFα detected in co-culture supernatant following 24 hours of 1:1 CD8⁺ T cell-to-U-2 OS ratio. *, P < 0.0005.