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September 30, 2025Transboundary and Emerging DiseasesOpen Access

A Multiplex TaqMan‐MGB qPCR Assay for Rapid and Accurate Identification of Four Waterfowl Parvoviruses (cGPV, MDPV, MDGPV, and SBDSV)

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Authors

MZMin ZhengDJDandan JiangSXShifeng Xiao

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Overview

This study develops a test for detecting waterfowl parvoviruses, improving surveillance and outbreak control.

Key Points

  • The multiplex TaqMan-MGB qPCR assay successfully detects four waterfowl parvoviruses with high specificity and sensitivity.
  • Detection limits were found at 10² copies/μL for cGPV and 10¹ copies/μL for MDPV, confirming its high performance.
  • Clinical testing with 337 samples showed the assay outperformed conventional PCR, achieving a detection rate of 58%.
  • Findings revealed ongoing cross-species transmission of waterfowl parvoviruses, with MDGPV identified in goslings for the first time.

Cite This Study

Zheng et al. (2025) studied this question.

synapsesocial.com/papers/68dc26268a7d58c25ebb313dhttps://doi.org/10.1155/tbed/9999490
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