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November 25, 2024MicroorganismsOpen Access

One-Step Multiplex Real-Time Fluorescent Quantitative Reverse Transcription PCR for Simultaneous Detection of Four Waterfowl Viruses

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Why the study?

Duck Tembusu virus, duck hepatitis virus, Muscovy duck reovirus, and Muscovy duck parvovirus impact waterfowl and are challenging to differentiate due to overlapping clinical signs.

Population

326 clinical specimens sourced from Guangxi

Design

Assay development and validation study

Authors

CWChenchen WangHLHuixin LiuJCJunze Cheng

Discussion

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Overview

May aid differential diagnosis of overlapping waterfowl infections; leaves open routine adoption pending field validation.

Structured PICO

P
Population
326 clinical specimens from waterfowl sourced from Guangxi
I
Intervention
One-step multiplex real-time fluorescence quantitative reverse transcription PCR (qRT-PCR) assay
O
Outcome
Simultaneous detection of duck Tembusu virus (DTMUV), duck hepatitis virus (DHV), Muscovy duck reovirus (MDRV), and Muscovy duck parvovirus (MDPV)surrogate

A novel multiplex qRT-PCR assay provides a highly specific, sensitive, and repeatable method for the simultaneous detection of four major waterfowl viruses.

Cite This Study

Wang et al. (2024) studied this question.

synapsesocial.com/papers/6a925b7669d1d358886763dfhttps://doi.org/10.3390/microorganisms12122423
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Also Consider

Synapse has enriched 5 closely related papers on similar clinical questions. Consider them for comparative context:

  1. 1Development and application of quadruplex real time quantitative PCR method for differentiation of Muscovy duck parvovirus, Goose parvovirus, Duck circovirus, and Duck adenovirus 32024 · 6 citations
  2. 2TaqMan-probe-based multiplex real-time qPCR assay for simultaneous detection of Duck Tembusu virus, Novel duck reovirus, and Duck circovirus2026
  3. 3Taqman-probe-based multiplex real-time RT-QPCR for simultaneous detection of duck tembusu virus, novel duck reovirus, duck hepatitis a type 1 virus and duck hepatitis a type 3 virus2025 · 10 citations
  4. 4Development of a Multiplex Quantitative Polymerase Chain Reaction Assay for the Detection of Duck Enteritis Virus, Goose Parvovirus, and Muscovy Duck Parvovirus2025 · 1 citations
  5. 5Development and application of multiplex PCR method for simultaneous detection of seven viruses in ducks2019 · 38 citations